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Senior Research Scientist

Eingestellt von Synectics

Gesuchte Skills: Support

Projektbeschreibung

JOB DESCRIPTION:
Research Scientist to provide support for shRNA/CRISPR reagent generation for projects that include:
1) Providing cell culture and molecular biology cloning support for shRNA/CRISPR vector construction, tracking of sequence confirmation and lentiviral shRNA packaging.
2) Providing high throughput lentiviral infection support and quantitation of virus titer.
3) Providing PCR support for genomic DNA characterization for NGS analysis on MiSeq platform.

RESPONSIBILITIES:

- Perform molecular biology support to generate vector specific shRNA and/or CRISPR clones to support projects.
- Track shRNA sequencing results and all inventory of all generated reagents using both LIMS and FreezerPro platforms.
- Provide support for 96 well high throughput lentiviral infection support for all shRNA/CRISPR clones using Biomek and Janus liquid handling equipment.
- Perform assays to quantitate Lentivirus titers and genomic DNA.
- Support development of new technologies and innovative approaches that aid in increased throughput or improvement in reagent quality.

SKILLS:

SKILLS/QUALIFICATIONS:

- Strong demonstrated experience and knowledge in molecular biology techniques including but not limited to cloning, PCR, plasmid purification and quantification.
- Experience in cell culture and lentiviral packaging, infection, and tittering of shRNA vectors.
- Expertise using semi-automated Biomek, Janus or similar liquid handling platforms.
- Experience in performing MiSeq or other NGS analysis would be beneficial but not required.
- Knowledge and/or experience in writing and or development of computer programs that track reagent processing functions on the liquid handling platforms.
- Masters preferred with minimum 3 years pharmaceutical experience, or undergraduate with 5-10 years experience.
- Prior work experience in industry is not required, but would be beneficial.
- Highly motivated and independent scientist who drives results to deliver on specific goals.
- Strong analytical thinker who strives to improve, innovate, and become a scientific leader.
- Detailed oriented and ability to perform multiple projects simultaneously with high efficiency.
- Highly knowledgeable in molecular biology and related shRNA vector and lentiviral characterization.
- Excellent communication and interpersonal skills.
- Works well in team environment.
- Strong technical knowledge with detailed assay skills and reproducible experimental techniques.
- Strong DNA and RNA molecular biology cloning experience.
- Demonstrated experience with RT-PCR and DNA sequencing analysis.
- Cell culture and experience working with lentivirus production and infection.
- Experience with robotic or liquid handling platforms would be beneficial.

Projektdetails

  • Einsatzort:

    Pearl River, Vereinigte Staaten

  • Projektbeginn:

    asap

  • Projektdauer:

    Keine Angabe

  • Vertragsart:

    Contract

  • Berufserfahrung:

    Keine Angabe

Geforderte Qualifikationen

  • Kategorie:

    Sonstiges

  • Skills:

    support

Synectics