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Neuroscience Postdoctoral Research
Eingestellt von Bio Careers
Gesuchte Skills: Support, Pure
Projektbeschreibung
An appointment is available at the U.S. Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD. This appointment includes, but is not limited to, the following:
· Research in the area of the ability to generate highly pure, large-scale cultures of homotypic glutamatergic neurons from suspension-cultured murine embryonic stem (ES) cells within eight days. ES cell-derived neurons (ESNs) express and localize neuron-specific proteins to appropriate axodendritic locations, form synapses, and release glutamate under depolarizing conditions; can be maintained in culture through at least 90 days after plating.
· ESNs are the first neuronal model demonstrated to be sensitive to all BoNT serotypes (/A-/G), with EC50s similar to primary mouse spinal cord neurons. Transgenic mESCs differentiate into recombinant neurons that continue to express genes of interest, allowing for the creation of reporter lines to facilitate BoNT research and drug discovery, and for the application of reverse genetics to evaluate the role of specific genes in the response to intoxication. Utilize this cell model to identify novel therapeutic approaches to rescue neurotransmission in intoxicated neurons, characterize the biochemistry of BoNT transport and activity, and apply systems biology approaches to evaluate the host response to BoNT intoxication.
· Support research that ESNs appear to have considerable utility as a cell-based model to research neurotoxins, neuropathogens, and environmental neuron injury. It has already been demonstrated that ESNs are susceptible to a second neurotoxin (black widow spider venom) and a publication describing the suitability of ESNs as a cell-based model of excitotoxicity is currently under review.
· Research the NMDA receptor in excitotoxic progression and identification of intracellular mechanisms of excitotoxicity that may be amenable to therapeutic intervention.
· Study transcriptional changes associated with neurogenesis and synaptogenesis, with the objective of identifying mechanisms of neuronal differentiation from a pluripotent stem cell population. Ultimately these efforts are directed towards development of a well-described neurotoxicological model for a variety of neuroactive agents.
· Utilize experience in cellular and molecular neuroscience , next generation sequencing and/or electrophysiology.
· Publish findings and support application for funding through DoD and NIH.
Projektdetails
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Einsatzort:
Aberdeen Proving Ground, Vereinigte Staaten
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Projektbeginn:
asap
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Projektdauer:
Keine Angabe
- Vertragsart:
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Berufserfahrung:
Keine Angabe
Geforderte Qualifikationen
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Kategorie:
IT Entwicklung, Sonstiges